JIOH on LinkedIn JIOH on Facebook
  • Users Online: 778
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL RESEARCH
Year : 2019  |  Volume : 11  |  Issue : 4  |  Page : 191-196

Cytotoxicity test of 4-methacryloxyethyl trimellitic anhydride–based dentine bonding material using acetone solution in dental pulp fibroblast


1 Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2 Resident in Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Dr. Adioro Soetojo
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jalan Prof. Dr. Moestopo No. 47, Surabaya 60132.
Indonesia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jioh.jioh_32_19

Rights and Permissions

Aims and Objectives: Both carious and non-carious lesions covering large dentine areas are indisputable indications of the need to use dentine bonding. Clinically, dental preparation, which is subsequently subjected to dentine bonding application often results in post-restorative pain. Various studies suggest that post-restorative pain is caused by the presence of residual monomers from the imperfect polymerization of a bonding material. The residual monomer can be a free radical that will induce oxidative stress conditions producing a toxic effect on 4-methacryloxyethyl trimellitic anhydride (4-META) monomer as the base material of dentine bonding. The aim of the study was to determine the toxic concentration of 4-META dentine bonding material using acetone as a solvent that destroys 50% of the dental pulp fibroblast cells. Materials and Methods: Human pulp fibroblast cells contained in each well were treated with 4-META-acetone solution at concentrations of 5000, 2500, 1250, 625, 312.5, 156.25, 78.12, 39.06, 19.53, and 9.76 μg/mL. Two wells were left untreated to form the control group. A cytotoxicity test was performed by means of an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-assay test. The optical density of each well was measured with an enzyme-linked immunosorbent assay (ELISA) reader and the percentage of human pulp fibroblast cell destroyed was calculated using the appropriate formula. Results: The concentration of 4-META-based dentine bonding with acetone solvent capable of causing 50% human pulp fibroblast cell death (LC50) was 1250 μg/mL. Conclusion: Toxic concentrations are those greater than or equal to 1250 μg/mL.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed16    
    Printed0    
    Emailed0    
    PDF Downloaded5    
    Comments [Add]    

Recommend this journal