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ORIGINAL RESEARCH
Year : 2019  |  Volume : 11  |  Issue : 7  |  Page : 40-43

The adhesion activity to monocytes against Streptococcus mutans with exposure steeping robusta coffee beans


1 Department of Biomedic, Faculty of Dentistry, Jember University, Jember, Jawa Timur, Indonesia
2 Department of Dental Public Health, Faculty of Dentistry, Jember University, Jember, Jawa Timur, Indonesia
3 Department of Pedodontics, Faculty of Dentistry, Jember University, Jember, Jawa Timur, Indonesia

Correspondence Address:
Prof. I Dewa Ayu Ratna
Jl. Kalimantan No. 37, Kampus Tegalboto, Jember, Jawa Timur
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jioh.jioh_245_18

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Aim and Objectives: The aim of the study is to analyze the adhesion activity to monocytes against Streptococcus mutans with exposure steeping robusta coffee beans in vitro. Materials and Methods: Peripheral blood sampling of healthy people 6 cc added anticoagulants. Peripheral blood sampling of healthy people 6 cc added anticoagulants and Ficoll-Hypaque, then centrifugation and suspended in medium Roswell Park Memorial Institute (RPMI) 1640. The cells were placed in 96-well microtiter plate 8 × 105 cells/well for 45 min at 37°C and washed 4 times with the medium. Attached is a monocyte. Furthermore, the isolate of monocytes and treated with the appropriate group. The control group – monocytes untreated; S. mutans group – monocytes + S. mutans; coffee group 2.5% – steeping coffee beans 2.5% + S. mutans; coffee group 5% – steeping coffee beans 5% + S. mutans; and coffee group 10% – steeping the coffee beans 10% + S. mutans. After incubated 24 h, fixed with methanol, performed Giemsa staining, and analyzed adhesion activity of under a light microscope With magnification 400 × on to 100 monocytes. Data were analyzed using analysis of variance (ANOVA) followed by least significant difference (LSD) test. Results: There were significant differences between the study groups. Steeping robusta coffee beans improve adhesion S. mutans in monocyte cells, while the largest adhesion at a concentration of 5%. Conclusions: Certain concentrations of steeping robusta coffee beans consumption increase adhesion activity to S. mutans.


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