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Year : 2020  |  Volume : 12  |  Issue : 3  |  Page : 270-274

Recombinant protein galectin-1 exposure to OM-1 cell proliferation: An experimental in vitro study

Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Dr. Andra Rizqiawan
Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo 47, Surabaya 60132.
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JIOH.JIOH_188_19

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Aim: The aim of this study was to overcome the shortage of galectin-1 (Gal-1) protein and to determine cell proliferation by adding recombinant protein that has a high protein content of the squamous cell OM-1. Materials and Methods: A presented study was Experimental laboratory study. The role of recombinant protein galectin‑1 (r‑Gal‑1) in combating squamous cell carcinoma of the OM‑1, as compared to HOC313 squamous cell carcinoma without exposure to r‑Gal‑1, through the promotion of cell proliferation. An in vitro experiment was conducted on the following three groups: a negative control group using OM-1 cells, an intervention group comprising OM-1 cells to which 8 µg/mL r-Gal-1 was added, and a positive control incorporating HOC313 cells that were highly invasive. The proliferation level of the cells was measured by means of the optical density on a microplate reader on days 0, 1, 2, 3, and 4. In the three test groups, an increased proliferation rate was obtained on days 1, 2, 3, and 4 on OM-1, OM-1 + r-Gal-1, and HOC313. The statistical analysis was carried out using analysis of variance test, Kruskal–Wallis test, least significant difference test, and Mann–Whitney test. Results: The proliferation rate increased significantly (P < 0.05) in the treatment group (OM-1 + r-Gal-1) as compared to the negative control group (OM-1). The positive control group (HOC313) experienced the highest degree of proliferation among the three groups. Conclusion: Gal-1 is a protein that plays a key role in the processes of cancer metastasis and invasion.

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