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Year : 2022  |  Volume : 14  |  Issue : 3  |  Page : 266-272

Evaluating the antibacterial efficacy and minimal bactericidal concentration (MBC) of three different herbal extracts on recalcitrant endodontic pathogens - An in vitro study

1 Department of Pediatric and Preventive Dentistry, SRM Kattankulathur Dental College, Chennai, India
2 Department of Public Health Dentistry, Ragas Dental College and Hospital, Uthandi, Chennai, India

Correspondence Address:
Dr. S Rajakumar
Department of Pediatric and Preventive Dentistry, SRM Kattankulathur Dental College and Hospital, Potheri
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jioh.jioh_5_22

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Aim: To evaluate the antibacterial efficacy, minimal inhibitory concentration, and minimal bactericidal concentrations of different herbal extracts against endodontic pathogens. Materials and Methods: The Microbiological samples were taken using sterile paper points from the root canals undergoing retreatment and samples were transferred into cultural plates containing selective media and incubated for 24hrs for the specified bacterial growth. The methanolic extracts of plant were obtained and stored in capped bottles at 4°C until use. The susceptibility of endodontic bacteria to the various plant extracts at different concentrations was analysed using disc diffusion method by measuring the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of the extracts were assessed to determine the efficient plant concentration against the resistant endodontic bacteria. Triplicates of all the tests were carried out. The average of three experiments were subjected to statistical analysis. Data was analysed using one way ANOVA using SPSS version 20.0 (Armonk. NY, USA) to find out the zone of inhibition of herbal extracts against microbiota. Results: Clitoria Ternatae exhibits significance (P ≤ 0.05) and higher inhibitory zones (30.33 ± 1.528, 30.43 ± 0.577, 20.33 ± 1.528) against all three micro pathogens. ClitoriaTernatea at its minimal concentration of 102 mg/ml is better against all the pathogens, the least being Veronica Officinalis (425 mg/ml). Conclusions: ClitoriaTernatea, showed better bactericidal action under lower concentrations against all pathogens followed by Altheae officinalis and Veronica officinalis.

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